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Catalog Number | orb149724 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal to VGlut1 (APC). VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | S28-9 |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG1 |
Immunogen | Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1 |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000) |
Conjugation | APC |
MW | 52kDa |
Target | VGLUT1 |
Entrez | 116638 |
UniProt ID | Q62634 |
NCBI | NP_446311.1 |
Storage | Conjugated antibodies should be stored at 4°C |
Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated |
Alternative names | BNPI antibody, SLC17A1 antibody, SLC17A1/VGLUT1 an Read more... |
Note | For research use only |
Application notes | 1 µg/ml of SMC-394 was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
WB | |
Animal, Bovine, Canine, Guinea pig, Human, Mouse, Rabbit, Rat | |
Canine, Equine, Guinea pig, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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