VEGFR2 (pY1054/Y1063) antibody

• Catalog Number: orb665503
• Category: Antibodies
• Description: Rabbit polyclonal antibody to VEGFR2 (pY1054/Y1063)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1054/Y1063 of human VEGFR2/3 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1000, IHC-P: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: KDR; FLT4
• Entrez: 3791, 114110, 25589, 14257, 2324
• UniProt ID: P35916, O08775, P35917, Q91ZT1, P35918, P35968
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-KDR antibody, anti-FLK1 antibody, anti-VEGFR2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of VEGFR2/3 (Phospho-Y1054/Y1063) expression in AML12 (A), PMVEC (B), rat brain (C) whole cell lysates. (Predicted band size: 151; 152 kD; Observed band size: 230 kD)

Immunohistochemical analysis of VEGFR2/3 (Phospho-Y1054/Y1063) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.