VEGF antibody

• Catalog Number: orb348842
• Category: Antibodies
• Description: Mouse monoclonal antibody to VEGF
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: A.4.6.1
• Tested applications: Blocking, ELISA, FC, IHC, IP, WB
• Reactivity: Human, Rat
• Isotype: IgG1
• Immunogen: Recombinant human VEGF.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: VEGF
• UniProt ID: P15692
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: VEGF-A; Vascular Endothelial Growth Factor; VEGFA;
• Note: For research use only
• Application notes: This is the parental antibody clone to the humanized, therapeutic antibody Bevacizumab (marketed as Avastin) binding to VEGF. VEGF is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels.
• Expiration Date: 12 months from date of receipt.

References

Kim, K. J. et al. The vascular endothelial growth factor proteins: identification of biologically relevant regions by neutralizing monoclonal antibodies Growth Factors, 7, 53-64 (1992)

PubMed: 1380254

IHC-P image of rat skin tissue using VEGF antibody (dilution of primary antibody at 5 ug/ml)

IHC-P staining of rat skin tissue using anti-VEGF (dilution at 5 ug/ml)

Immunohistochemical staining of paraffin embedded human breast cancer tissue using VEGF antibody (primary antibody at 5 ug/ml)

Immunohistochemical staining of paraffin embedded rat bladder tissue using VEGF antibody (primary antibody at 5 ug/ml)

Immunohistochemical staining of rat brain tissue using anti-VEGF (dilution of primary antibody - 5 ug/ml)

Western blot analysis of rat skin (lane 1), mouse brain (lane 2), rat bladder (lane 3), human breast caner (lane 4); Hels cells (lane 5) using VEGF antibody (1 ug/ml)