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Catalog Number | orb1806548 |
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Category | Assays and Kits |
Description | VD3(Vitamin D3) ELISA Kit |
Reactivity | All |
Range | 6.25-400 ng/mL |
Target | VD3 |
Sensitivity | 2.14 ng/mL |
Alternative names | Cholecalciferol; Toxiferol; Activated 7-Dehydrocho Read more... |
Note | For research use only |
Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin D3(VD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin D3(VD3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D3(VD3) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Sample Types | serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
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