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VCP rabbit pAb

Catalog Number: orb770367

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb770367
CategoryAntibodies
DescriptionRabbit polyclonal antibody to VCP
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Concentration1 mg/ml
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
ImmunogenThe antiserum was produced against synthesized peptide derived from human VCP. AA range:318-367
MW85
Tested applicationsELISA, IHC-P, WB
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAnti-VCP antibody, anti-Transitional endoplasmic r
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NoteFor research use only
VCP rabbit pAb

Western Blot analysis of K562 cells using VCP Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000.

VCP rabbit pAb

Western blot analysis of lysates from NIH/3T3 cells, treated with starved 24h, using VCP Antibody. The lane on the right is blocked with the synthesized peptide.

VCP rabbit pAb

Western blot analysis of the lysates from HepG2 cells using VCP antibody.

VCP rabbit pAb

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

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