VCAM1 Antibody

• Catalog Number: orb1671611
• Category: Antibodies
• Description: VCAM1 Antibody
• Clonality: Recombinant
• Clone Number: B-K9 (C106-K9)
• Tested applications: Blocking, FC
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunizing Balb/c mice against activated human umbilical vein endothelial cells (HUVEC).
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: VCAM1
• UniProt ID: P19320
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: VCAM1, Vascular cell adhesion protein 1, V-CAM 1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-CD106 antibody B-K9 (C106-K9). Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix™ coverslips stained with the chimeric r version of B-K9 (C106-K9) (orb1671611) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671611- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-CD106 antibody B-K9 (C106-K9). Paraformaldehyde fixed K562 cells were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of B-K9 (C106-K9) (orb1671611 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.