VAPB Antibody

• Catalog Number: orb413051
• Category: Antibodies
• Description: VAPB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human VAPB recombinant protein (Position: A2-R55). Human VAPB shares 98.1% and 100% amino acid (aa) sequence identity with mouse and rat VAPB, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry|1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 27 kDa
• UniProt ID: O95292
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Vesicle-associated membrane protein-associated pro
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of CACO-2 cells using anti-VAPB antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U87 cells using anti-VAPB antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of VAPB using anti-VAPB antibody.Lane 1:human HeLa cell; 2:human MCF-7 cell; 3:human COLO-320 cell; 4:human HepG2 cell;5:human PANC-1 cell;6:human A375 cell;7:human Jurkat cell;8:human placenta tissue.

WB analysis of VAPB using anti-VAPB antibody.Lane 1:mouse liver tissue; 2:mouse heart tissue; 3:mouse skeletal muscle tissue; 4:mouse HEPA1-6 cell.

IF analysis of VAPB using anti-VAPB antibody. VAPB was detected in immunocytochemical section of A431 cells.

IHC analysis of VAPB using anti-VAPB antibody. VAPB was detected in paraffin-embedded section of human renal cancer tissue.

IHC analysis of VAPB using anti-VAPB antibody. VAPB was detected in paraffin-embedded section of human liver cancer tissue.

IHC analysis of VAPB using anti-VAPB antibody. VAPB was detected in paraffin-embedded section of mouse lung tissue.

IHC analysis of VAPB using anti-VAPB antibody. VAPB was detected in paraffin-embedded section of human renal cancer tissue.