VAP-33 Antibody

• Catalog Number: orb1523127
• Category: Antibodies
• Description: VAP-33 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 125 and 175
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: VAP-33
• UniProt ID: Q9P0L0
• NCBI: NP_919415.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: VAMP-A; VAP-A; VAP-33; vesicle-associated membrane
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse VAP-33 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN or RIPA buffer from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-VAP-33 antibody orb1523127 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human VAP-33 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-VAP-33 antibody orb1523127 used for IP at 6 µg per reaction.

Detection of human VAP-33 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-VAP-33 (Cat. No. orb1523127) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB