VAChT Antibody (Biotin)

SKU: orb149708

Description

Mouse monoclonal to VAChT (Biotin). VAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicle for release into the extracellular space. Acetylcholine (Ach) transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene..

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution range: WB (1:1000), IHC (1:200), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A dilution of 1:50-1:200 of SMC-393 was sufficient for detection of VAChT Transporter in rat brain using immunohistochemistry analysis and goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG1
• Clone No.: S6
• Immunogen: Synthetic peptide amino acids 521-532 of human VAChT
• Target: VAChT
• Molecular Weight: 56kDa
• Purification: Protein A Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Vesicular Acetylcholine Transporter, VAChT, SLC18A3, Slc18a3, Solute carrier family 18 member 3, Solute carrier family 18 (vesicular acetylcholine) member 3, Solute carrier family 18 (vesicular monoamine) member 3, Vesamicol-binding protein, MGC12716, rVAT, Unc-17

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone N6/38. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VAChT Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone N6/38. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VAChT Antibody. (D) Composite.

Western Blot analysis of Rat brain membrane lysate showing detection of VAChT protein using Mouse Anti-VAChT Monoclonal Antibody, Clone N6/38. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

VAChT

UniProt

Q16572

NCBI Gene ID

6572

RefSeq (Protein):NP_003046.2