USP1 Antibody

• Catalog Number: orb1527948
• Category: Antibodies
• Description: USP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP
• Reactivity: Human
• Immunogen: between 450 and 500
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. A301-699A has not performed satisfactorily when used for WB of USP1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of USP1.; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: USP1
• UniProt ID: O94782
• NCBI: NP_003359.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: ubiquitin specific processing protease 1; Ubiquiti
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human USP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP1 antibody orb1527948 used for IP at 6 µg per reaction.

Detection of human USP1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-USP1 (Cat. No. orb1527948) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB