USF1 monoclonal antibody (M01), clone 3F6

• Catalog Number: orb2291983
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant USF1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3F6
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG1 Kappa
• Immunogen: USF1 (AAH35505, 1 a.a. ~ 310 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN
• NCBI: AAH35505
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged USF1 is approximately 0.03 ng/ml as a capture antibody.

USF1 monoclonal antibody (M01), clone 3F6 Western Blot analysis of USF1 expression in K-562.

Western Blot analysis of USF1 expression in transfected 293T cell line by USF1 monoclonal antibody (M01), clone 3F6. Lane 1: USF1 transfected lysate (33.5 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of USF1 over-expressed 293 cell line, cotransfected with USF1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with USF1 monoclonal antibody (M01), clone 3F6 (Cat # orb2291983). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (59.84 KDa).