UNG Antibody

• Catalog Number: orb1238581
• Category: Antibodies
• Description: UNG Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-UNG1 antibody (orb1238581) was raised against a peptide corresponding to 14 amino acids near the center of human UNG1. The immunogen is located within amino acids 200-250 of UNG1.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-4 μg/mL; IHC: 2 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 34kDObserved: 34 kD
• Target: UNG
• UniProt ID: P13051
• NCBI: NP_003353
• Storage: UNG1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: UNG1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: UNG1 Antibody: DGU, UDG, UNG1, UNG2, HIGM4, HIGM5,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Mouse C2C12 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: UNG1, orb1238581 (A: 0.5 µg/mL, B: 1 µg/mL and C: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: UNG1, orb1240273 (0.5 µg/mL), UNG1, orb1238581 (4 µg/mL), beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of UNG1 in Human Heart Tissue. Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-UNG1 antibody (orb1238581) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of UNG1 in Human Heart Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human heart labeling UNG1 with orb1238581 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).