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Catalog Number | orb1270331 |
---|---|
Category | Antibodies |
Description | UNCX Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This UNCX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the C-terminal region of human UNCX. |
Concentration | batch dependent |
Dilution range | For IF starting dilution is: 1:25For WB starting dilution is: 1:1000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 54 kDa |
Target | UNCX |
UniProt ID | A6NJT0 |
NCBI | A6NJT0 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Homeobox protein unc-4 homolog, Homeobox protein U Read more... |
Note | For research use only |
Application notes | For IF starting dilution is: 1:25For WB starting dilution is: 1:1000 |
Expiration Date | 12 months from date of receipt. |
Immunofluorescent analysis of U251 cells, using UNCX Antibody. Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Western blot analysis in CEM cell line lysates (35 ug/lane).
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