ULK3 Antibody

• Catalog Number: orb308814
• Category: Antibodies
• Description: ULK3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at C-terminus of human ULK3 (427-454aa HTEVQNLMARAEYLKEQVKMRESRWEAD), different from the related mouse and rat sequences by four amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 53444 MW
• UniProt ID: Q6PHR2
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Serine/threonine-protein kinase ULK3;2.7.11.1;Unc-
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-ULK3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of THP-1 cells using anti-ULK3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ULK3 using anti-ULK3 antibody.Lane 1:Rat Brain Tissue;2:Rat Testis Tissue;3:Mouse Brain Tissue;4:Human Placenta Tissue;5:22RV1 Cell;6:SMMC Cell.

IHC analysis of ULK3 using anti-ULK3 antibody. ULK3 was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of ULK3 using anti-ULK3 antibody. ULK3 was detected in a paraffin-embedded section of rat brain tissue.