UBQLN1 Antibody

• Catalog Number: orb1520226
• Category: Antibodies
• Description: UBQLN1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 20-40 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: UBQLN1
• UniProt ID: Q9UMX0
• NCBI: Q9UMX0.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: ubiquilin-1; PLIC-1; XDRP1; UBQN; DA41; testicular
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human UBQLN1 by western blot. Samples: Whole cell lysate (10 µg) from RKO, CCRF-CEM, MCF-7, MDA-MB-435, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBQLN1 antibody (orb1520226) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse UBQLN1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBQLN1 antibody (orb1520226) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human UBQLN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBQLN1 antibody (orb1520226) used for IP at 1 µg per reaction.