UBN1 Antibody

• Catalog Number: orb1248665
• Category: Antibodies
• Description: UBN1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat, Zebrafish
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DNSEAYDELVPASLT
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 120 kDa
• Target: UBN1
• UniProt ID: Q9NPG3
• NCBI: NP_058632.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: UBN1, ubinuclein 1, VT, VT4, HIRA-binding protein,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 120kDa band observed in lysates of cell line MOLT4 (calculated MW of 122kDa according to NP_058632.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Expiration Date: 12 months from date of receipt.

orb1248665 (1 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.