Ubiquitin/UBB Antibody

• Catalog Number: orb196292
• Category: Antibodies
• Description: Ubiquitin/UBB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Predicted Reactivity: Bovine, Canine, Drosophila, Equine, Gallus, Hamster, Monkey, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Ubiquitin recombinant protein (Position: M77-G152).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 25762 MW
• UniProt ID: P0CG47
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Polyubiquitin-B;Ubiquitin;UBB;
• Note: For research use only
• Application notes: WB: The detection limit for Ubiquitin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Ubiquitin using anti-Ubiquitin antibody.Lane 1:human Caco-2 cell;2:human K562 cell;3:human THP-1 cell;4:rat PC-12 cell;5:rat brain tissue;6:mouse brain tissue.

IF analysis of Ubiquitin using anti-Ubiquitin antibody. Ubiquitin was detected in immunocytochemical section of A431 cells.

IHC analysis of Ubiquitin using anti-Ubiquitin antibody.Ubiquitin was detected in paraffin-embedded section of rat intestine tissue.

IHC analysis of Ubiquitin using anti-Ubiquitin antibody.Ubiquitin was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of Ubiquitin using anti-Ubiquitin antibody.Ubiquitin was detected in paraffin-embedded section of mouse intestine tissue.