UBE3A antibody

• Catalog Number: orb340776
• Category: Antibodies
• Description: Rabbit polyclonal antibody to UBE3A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human E6AP
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: UBE3A
• Entrez: 7337
• UniProt ID: O08759, Q05086
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: E6AP; EPVE6AP; HPVE6A; Ubiquitin-protein ligase E3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of E6AP expression in SKOV3 (A), Jurkat (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 97; 100 kD; Observed band size: 110 kD)

Immunohistochemical analysis of E6AP staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.