UBE3A antibody

• Catalog Number: orb131783
• Category: Antibodies
• Description: Rabbit polyclonal to UBE3A. Ubiquitin-protein ligase E3A (UBE3A) is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. UBE3A catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, UBE3A may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Immunogen: N-terminal region of human UBE3A
• Concentration: 100 μg/ml BSA
• Dilution range: WB:1:1000-1:3000
• Purity: Affinity purification
• Conjugation: Unconjugated
• MW: 101
• UniProt ID: Q05086
• Storage: -20°C
• Buffer/Preservatives: 0.01% NaN3
• Alternative names: anti Ubiquitin-protein ligase E3A antibody, anti E
• Note: For research use only
• Application notes: WB: The apparent protein size on WB may be different from the calculated M.W. due to modifications.; ; Experiment Notes: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human UBE3A. Antibodies were purified by affinity purification using immunogen.
• Expiration Date: 12 months from date of receipt.