UBE2R2 antibody

• Catalog Number: orb382107
• Category: Antibodies
• Description: Rabbit polyclonal antibody to UBE2R2.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human CDC34B
• Dilution range: WB: 1:500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: UBE2R2
• Entrez: 54926, 67615
• UniProt ID: Q6ZWZ2, Q712K3
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: CDC34B; UBC3B; Ubiquitin-conjugating enzyme E2 R2;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CDC34B expression in Jurkat (A), MCF7 (B), mouse liver (C), mouse kidney (D), rat brain (E), rat lung (F) whole cell lysates. (Predicted band size: 27 kD; Observed band size: 27 kD)

Immunofluorescent analysis of CDC34B staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).