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    UBE2J2 antibody

    Catalog Number: orb341274

    DispatchUsually dispatched within 5-10 working days
    $ 413.00
    Catalog Numberorb341274
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to UBE2J2
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Rat
    ImmunogenRecombinant full length protein of human UBE2J2
    Dilution rangeWB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:100
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetUBE2J2
    Entrez118424, 140499
    UniProt IDQ8N2K1, Q6P073
    SourceRabbit
    StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesNCUBE2; Ubiquitin-conjugating enzyme E2 J2; Non-ca
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    UBE2J2 antibody

    Western blot analysis of UBE2J2 expression in Hela (A), mouse testis (B), mouse brain (C) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 28 kD)

    UBE2J2 antibody

    Immunohistochemical analysis of UBE2J2 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    UBE2J2 antibody

    Immunofluorescent analysis of UBE2J2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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      Rabbit

      Polyclonal

      Unconjugated

      100 μl
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      ELISA,  IP,  WB

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      Polyclonal

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      ELISA,  IP,  WB

      Human, Mouse, Rat

      Rabbit

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