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    UBE2F antibody

    Catalog Number: orb412329

    DispatchUsually dispatched within 5-10 working days
    $ 413.00
    Catalog Numberorb412329
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to UBE2F
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Rat
    ImmunogenRecombinant full length protein of human UBE2F
    Dilution rangeWB: 1:500-2000
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetUBE2F
    Entrez363284, 140739
    UniProt IDQ969M7, Q5U203
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesanti-NCE2 antibody, anti-NEDD8-conjugating enzyme
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    UBE2F antibody

    Western blot analysis of UBE2F expression in K562 (A), mouse liver (B), mouse heart (C) whole cell lysates. (Predicted band size: 11; 13; 17; 18; 21; 23 kD; Observed band size: 21 kD)

    UBE2F antibody

    Immunohistochemical analysis of UBE2F staining in rat ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    UBE2F antibody

    Immunofluorescent analysis of UBE2F staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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      ELISA,  IHC,  WB

      Bovine, Canine, Rat

      Human, Mouse

      Goat

      Polyclonal

      Unconjugated

      0.1 mg
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