UBE2B Antibody

• Catalog Number: orb1264989
• Category: Antibodies
• Description: UBE2B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Bovine, Mouse, Rabbit, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This UBE2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the C-terminal region of human UBE2B.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17 kDa
• Target: UBE2B
• UniProt ID: P63146
• NCBI: P63146
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Ubiquitin-conjugating enzyme E2 B, RAD6 homolog B,
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of UBE2B Antibody in Ramos cell line lysates (35 ug/lane)

Western blot analysis of UBE2B using rabbit polyclonal UBE2B Antibody (E132) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2B gene.