UBA52 (phospho-S65) antibody

• Catalog Number: orb515510
• Category: Antibodies
• Description: Rabbit polyclonal antibody to UBA52 (phospho-S65)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S65 of human Ubiquitin protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: UBA52; RPS27A; UBB; UBC
• Entrez: 7311, 7314, 7316, 6233
• UniProt ID: P62987, P0CG47, P62979, P0CG48
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: UBB; Polyubiquitin-B; UBC; Polyubiquitin-C; RPS27A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Ubiquitin (Phospho-S65) expression in C6 (A), AML12 (B), SGC7901-UV treated (C), SGC7901 (D) whole cell lysates. (Predicted band size: 25; 77; 17; 14 kD; Observed band size: 15; more kD)

Direct ELISA antibody dose-response curve using Anti-Ubiquitin (Phospho-S65) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.