UBA1 Antibody

• Catalog Number: orb1264991
• Category: Antibodies
• Description: UBA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Bovine, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This E1 Ubiquitin (UBE1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1026-1058 amino acids from the C-terminal region of human E1 Ubiquitin (UBE1).
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 118 kDa
• Target: UBA1
• UniProt ID: P22314
• NCBI: P22314
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Ubiquitin-like modifier-activating enzyme 1, Prote
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of E1 Ubiquitin (UBE1) Antibody in mouse stomach tissue lysates (35 ug/lane)

Antibody is used in Western blot to detect UBE1 in HL-60 cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with UBE1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.