U2AF35 Antibody

• Catalog Number: orb1527260
• Category: Antibodies
• Description: U2AF35 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 140 and 190
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: U2AF35
• UniProt ID: Q01081
• NCBI: NP_006749.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: splicing factor U2AF 35kDa subunit; U2AFBP; U2AF35
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse U2AF35 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-U2AF35 antibody (orb1527260) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human U2AF35 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, K-562, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-U2AF35 antibody (orb1527260) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human U2AF35 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-U2AF35 antibody (orb1527260) used for IP at 6 µg per reaction. U2AF35 was also immunoprecipitated by a previous lot of this antibody (orb1527260) and a second antibody against a different epitope of U2AF35.

Detection of mouse U2AF35 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- U2AF35 (Cat. No. orb1527260) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human U2AF35 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- U2AF35 (orb1527260) used at a dilution of 1: 200 (1µg/ml). Detection: DAB