TXN Antibody

• Catalog Number: orb1256282
• Category: Antibodies
• Description: TXN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 20 to the C-terminus of human TXN (NP_003320.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 12kDa
• Target: TXN
• UniProt ID: P10599
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: TXN, ADF, ATL-derived factor, SASP, Thioredoxin, T
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using TXN antibody (orb1256282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse lung using TXN antibody (orb1256282) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 150 ug extracts of MCF7 cells using 3 ug TXN antibody (orb1256282). Western blot was performed from the immunoprecipitate using TXN antibody (orb1256282) at a dilution of 1:500.