Tubulin beta 3 antibody (FITC)

• Catalog Number: orb44545
• Category: Antibodies
• Description: Mouse monoclonal antibody against Tubulin beta 3 conjugated to FITC
• Clonality: Monoclonal
• Clone Number: TU-20
• Tested applications: FC, ICC
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Isotype: Mouse IgG1
• Immunogen: Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-3 μg/ml. Intracellular staining.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: betaIII-Tubulin
• Entrez: 10381
• UniProt ID: Q13509
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti-beta 3 tubulin antibody, anti-beta-4 antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-3 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of MCF-7 cells stained using anti-human betaIII-Tubulin (TU-20) FITC antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from MCF-7 cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 15 μg/ml, same as betaIII-Tubulin FITC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of MCF-7 cell suspension.

Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 FITC, green, 1 μg/ml). Nuclei were stained with DAPI (blue).