TUBA4A antibody

• Catalog Number: orb340710
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TUBA4A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human Alpha-tubulin 4a
• Dilution range: WB: 1:500-1:2000, IF/ICC: 1:10-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: TUBA4A
• Entrez: 7277, 22145, 316531
• UniProt ID: P68368, P68366, Q5XIF6
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: TUBA1; Tubulin alpha-4A chain; Alpha-tubulin 1; Te
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Alpha-tubulin 4a expression in LOVO (A), HEK293T (B), HUVEC (C) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 52 kD)

Immunofluorescent analysis of Alpha-tubulin 4a staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.