TUBA1 antibody

• Catalog Number: orb738510
• Category: Antibodies
• Description: Mouse monoclonal antibody to TUBA1
• Clonality: Monoclonal
• Clone Number: YOL1/34
• Tested applications: ELISA, FC, ICC, IHC-Fr, IHC-P, WB
• Reactivity: Aves, Human, Mammal, Mouse, Rat, Yeast
• Isotype: Rat IgG2a
• Immunogen: Yeast tubulin
• Concentration: 1 mg/ml
• Dilution range: ICC: 4-8 μg/ml, WB: 1 μg/ml
• Purity: Purified by protein-G affinity chromatography.
• Conjugation: Unconjugated
• Target: alpha-Tubulin
• Entrez: 7277
• UniProt ID: Q71U36
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-TUBA antibody
• Note: For research use only
• Application notes: Immunocytochemistry: Recommended dilution 4-8 μg/ml.Western blotting: Recommended dilution 1-2 μg/ml, reducing conditions.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry staining of alpha-tubulin in K562 cells using purified rat monoclonal antibody YOL1/34 (concentration in sample 6 μg/ml, DAR FITC, left picture) vs. Hoechst 34580 nuclear staining (right picture).

Western blotting analysis of human alpha-tubulin using rat monoclonal antibody YOL1/34 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rat anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated anti-rat secondary antibody. A specific band was detected for alpha-tubulin at approximately 54 kDa.

Western blotting analysis of human alpha-tubulin using rat monoclonal antibody YOL1/34 on lysates of Caenorhabditis elegans, Arabidopsis thaliana, Trypanosoma brucei, Drosophila melanogaster, human HeLa cell line, and porcine brain, all under reducing conditions. Each lane contains 20 μg of total protein. Nitrocellulose membrane was probed with 2 µg/ml of rat anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated anti-rat secondary antibody.