TTN monoclonal antibody (M09), clone 6H5

• Catalog Number: orb2292012
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant TTN.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: TTN (AAH58824, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MTTQAPTFTQPLQSVVVLEGSTATFEAHISGFPVPEVSWFRDGQVISTSTLPGVQISFSDGRAKLTIPAVTKANSGRYSLKATNGSGQATSTAELLVKAETAPPNFVQRL
• Tested applications: ELISA, IF, IHC-P, WB
• Clone Number: 6H5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH58824

Detection limit for recombinant GST tagged TTN is approximately 0.03 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to TTN on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to TTN on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]

Western Blot analysis of TTN expression in transfected 293T cell line by TTN monoclonal antibody (M09), clone 6H5. Lane 1: TTN transfected lysate (32.8 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (37.84 KDa).