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Catalog Number | orb1520304 |
---|---|
Category | Antibodies |
Description | TTC37 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | between 1 and 50 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 20-40 µl/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | TTC37 |
UniProt ID | Q6PGP7 |
NCBI | Q6PGP7.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | Ski3; thespin; tricho-hepatic-enteric syndrome pro Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse TTC37 by western blot. Samples: Whole cell lysate (50 µg) from GaMG, HEK293T, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-TTC37 antibody (orb1520304) used for WB at 0.1 mg/ml. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time time of 30 seconds.
Detection of human TTC37 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from GaMG cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-TTC37 antibody (orb1520304) used for IP at 2 µg per reaction.
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