TSPYL1 Antibody

• Catalog Number: orb1261036
• Category: Antibodies
• Description: TSPYL1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human TSPYL1 (NP_003300.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 60kDa
• Target: TSPYL1
• UniProt ID: Q9H0U9
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Testis-specific Y-encoded-like protein 1, TSPY-lik
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using TSPYL1 antibody (orb1261036) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug TSPYL1 antibody (orb1261036). Western blot was performed from the immunoprecipitate using TSPYL1 antibody (orb1261036) at a dilution of 1:1000.