TSPAN1 Antibody

• Catalog Number: orb1671657
• Category: Antibodies
• Description: TSPAN1 Antibody
• Clonality: Recombinant
• Clone Number: 4/12
• Tested applications: ELISA, IF, IHC
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising BALB/c mice with the major extracellular domain of tetraspanin 1.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: TSPAN1
• UniProt ID: O60635
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Net1, C4.8, Tetraspanin-1, Tspan-1, TSPAN1, Tetras
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed A431 cells with anti-Tetraspanin 1 antibody 4/12. Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric r version 4/12 (orb1671657) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671657- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-Tetraspanin 1 antibody 4/12. Paraformaldehyde fixed A431 cells permewith 0.5% triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of 4/12 (orb1671657 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.