TSC2 Antibody

SKU: orb1440594

Description

Mouse monoclonal antibody to TSC2.

Research Area

Cell Biology, Infectious Disease & Virology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF: 1:10~50, WB: 1:100
• Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: 249CT19.1.3
• Immunogen: Recombinant Protein
• Target: TSC2
• Molecular Weight: 200608
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tuberin, Tuberous sclerosis 2 protein, TSC2, TSC4

Fluorescent image of Hela cell stained with TSC2 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSC2 primary antibody (1:25, 1 h at 37°C. For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C. Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). TSC2 immunoreactivity is localized to Microtubules significantly.

TSC2 Antibody western blot analysis in mouse NIH-3T3 cell line lysates (35 μg/lane). This demonstrates the TSC2 antibody detected the TSC2 protein (arrow).

Quick Database Links

Gene Symbol

TSC2

UniProt

P49815

RefSeq (Protein):NP_000539.2, NP_001107854.1, NP_001070651.1