Trx-Tag Mouse Monoclonal Antibody

SKU: orb500892

Description

Trx-Tag Mouse Monoclonal Antibody

Research Area

Oxidative Stress

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, ELISA=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000, ICC/IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: All
• Predicted Reactivity: Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: B9I32
• Immunogen: Recombinant human Thioredoxin
• Target: TXN
• Molecular Weight: 35 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TRDX; TRX; TRX1; TXN1; Trx80; ADF; Txn; THIO_HUMAN; ATL-derived factor (ADF); Surface-associated sulphydryl protein (SASP); THIO_MOUSE; THIO_RAT;

Sample: Lane 1: Recombined Trx/His-tag porcine GM-CSF protein (Aa18-144) at 2 ug Lane 2: Enzyme digested Trx-tag Primary: Anti-Trx-Tag (orb500892) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 33/12 kD Observed band size: 33/12 kD.

Sample: Lane 1: Trx-tagged fusion protein (32-186) Overexpression E.coli Lysate at 2 ug, Lane 2: Trx-tagged fusion protein (19-213) Overexpression E.coli Lysate at 2 ug, Lane 3: Trx-tagged fusion protein (20-152) Overexpression E.coli Lysate at 2 ug, Primary: Anti-Trx-Tag (orb500892) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 30-40 kD, Observed band size: 30-40 kD.

Quick Database Links

Gene Symbol

TXN

UniProt

P10599