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Catalog Number | orb1526156 |
---|---|
Category | Antibodies |
Description | TRPM7 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 1815 and 1865 |
Concentration | 200 µg/ml |
Dilution range | WB - 1:200 - 1:1,000; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | TRPM7 |
UniProt ID | Q96QT4 |
NCBI | NP_060142.3 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | LTRPC7; TRP-PLIK; transient receptor potential-pho Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human TRPM7 by western blot. Samples: Whole cell lysate (35 µg) from K-562, LNCaP, Hep-G2, RKO, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRPM7 antibody (orb1526156) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human TRPM7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRPM7 antibody (orb1526156) used for IP at 6 µg per reaction. TRPM7 was also immunoprecipitated by a previous lot of this antibody (orb1526156) and a second antibody against a different epitope of TRPM7.
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