TrpC7 Antibody: Biotin

• Catalog Number: orb148858
• Category: Antibodies
• Description: Mouse monoclonal to TrpC7 (Biotin). Transient receptor potential cation channel, subfamily C, member 7, also known as TRPC7, is a non-selective cation channel that is directly activated by DAG. TrpC7 shows constitutive activity and susceptibility to negative regulation by extracellular Ca2+. Because of this, TrpC7 plays an important role in the Ca2+ signaling pathway. TrpC7 is also expressed abundantly in the heart, and combined with its ability to act as a Ca2+ channel, TrpC7 might contribute to the process of heart failure..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N64A/36 (Formerly sold as S64A-36)
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Synthetic peptide amino acids 845-862 of human TRPC7
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC (1:1000), ICC/IF (1:100)
• Conjugation: Biotin
• MW: 100kDa
• Target: TRPC7
• Entrez: 57113
• UniProt ID: Q9HCX4
• NCBI: NP_065122.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: TRP7 antibody, KNP3 antibody, TRPM2 antibody, tran
• Note: For research use only
• Application notes: 1 µg/ml of SMC-343 was sufficient for detection of TrpC7 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC7 Antibody (D) Composite.

Immunohistochemistry analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything.

Western Blot analysis of Rat brain membrane lysate showing detection of TrpC7 protein using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear staining.