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Item 1 of 4
Item 1 of 4
TrpC7 Antibody: APC
Catalog Number: orb148857
| Catalog Number | orb148857 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal to TrpC7 (APC). Transient receptor potential cation channel, subfamily C, member 7, also known as TRPC7, is a non-selective cation channel that is directly activated by DAG. TrpC7 shows constitutive activity and susceptibility to negative regulation by extracellular Ca2+. Because of this, TrpC7 plays an important role in the Ca2+ signaling pathway. TrpC7 is also expressed abundantly in the heart, and combined with its ability to act as a Ca2+ channel, TrpC7 might contribute to the process of heart failure.. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | N64A/36 (Formerly sold as S64A-36) |
| Tested applications | ICC, IF, IHC |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG1 |
| Immunogen | Synthetic peptide amino acids 845-862 of human TRPC7 |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000), IHC (1:1000), ICC/IF (1:100) |
| Conjugation | APC |
| MW | 100kDa |
| Target | TRPC7 |
| Entrez | 57113 |
| UniProt ID | Q9HCX4 |
| NCBI | NP_065122.1 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Alternative names | TRP7 antibody, KNP3 antibody, TRPM2 antibody, tran Read more... |
| Note | For research use only |
| Application notes | 1 µg/ml of SMC-343 was sufficient for detection of TrpC7 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC7 Antibody (D) Composite.
Immunohistochemistry analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything.
Western Blot analysis of Rat brain membrane lysate showing detection of TrpC7 protein using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear staining.
