Troponin I Antibody

• Catalog Number: orb1806455
• Category: Antibodies
• Description: Troponin I Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Immunogen: between 1 and 50
• Concentration: 250 µg/ml
• Dilution range: WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Troponin I
• UniProt ID: P19429
• Storage: Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: RCM1; troponin I, cardiac muscle; troponin I type
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Troponin I by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, heart, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Troponin I recombinant monoclonal antibody (orb1806455) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-GAPDH antibody.

Detection of mouse Troponin I by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, heart, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Troponin I recombinant monoclonal antibody (orb1806455) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-GAPDH antibody.

Detection of human Troponin I by immunohistochemistry. Sample: FFPE section of human cardiac muscle. Antibody: Rabbit anti-Troponin I recombinant monoclonal antibody (orb1806455). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse Troponin I by immunohistochemistry. Sample: FFPE section of mouse cardiac muscle. Antibody: Rabbit anti-Troponin I recombinant monoclonal antibody (orb1806455). Secondary: HRP-conjugated goat anti-rabbit IgG.