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Catalog Number | orb1958831 |
---|---|
Category | Proteins |
Description | TrkB Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.7 kDa and the accession number is Q16620-2. |
Tag | C-His |
Purity | 99.30% |
MW | 45.7 kDa (predicted); 70-80 kDa (reducing condition, due to glycosylation) |
UniProt ID | Q16620 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | TrkB Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.7 kDa and the accession number is Q16620-2. |
Expression Region | A DNA sequence encoding the extracellular domain (Met 1-His 430) of human TrkB (NP_001007098.1) precursor was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Cys 32 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.0 kDa after removal of the signal peptide. The apparent molecular mass of NTRK2-His is approximately 55-100 kDa due to glycosylation. | |
Mammalian |
45.66 kDa (predicted); 65.4 kDa (reducing contition, due to glycosylation) |
> 97% by SDS-PAGE. | |
Recombinant Human TrkB Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Cys32-His Tag430) of human TrkB (Accession #NP_001018074.1) fused with a 6��His Tag at the C-terminus. |