Triptolide

SKU: orb1306199

Description

Triptolide

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 38748-32-2
• MW: 360.40
• Purity: 99.97%
• Formula: C₂₀H₂₄O₆
• SMILES: [H][C@@]12C[C@@H]3O[C@@]33[C@H](O)[C@]4(O[C@H]4[C@@H]4O[C@]34[C@@]1(C)CCC1=C2COC1=O)C(C)C
• Target: NF-κB,Apoptosis,HSP,Mdm2
• Solubility: DMSO:51.00 mg/mL (141.51 mM)

Bioactivity

• Target IC50:
  MDM2-p53:47-73 nM (IC50)
• In Vivo:
  METHODS: To assay antitumor activity in vivo, Triptolide (0.4 mg/kg) was administered by gavage to BALB/c-nu+/nu+ mice harboring the breast cancer tumor MCF-7 once daily for three weeks. RESULTS: Triptolide inhibited the growth of MCF-7 cell xenografts in a mouse model. METHODS: To investigate the effects on ulcerative colitis (UC), Triptolide (0.1-0.4 mg/kg) was administered orally to DSS-induced UC mice once daily for seven days. RESULTS: Triptolide has anti-inflammatory and therapeutic effects on UC mice.
• In Vitro:
  METHODS: Neuroblastoma cells BE(2)-C were treated with Triptolide (5-100 nM) for 24 h. Cell viability was measured using the CCK-8 Assay. RESULTS: A concentration-dependent response to Triptolide was observed in BE(2)-C cells. At 50 nM Triptolide, cell viability was significantly reduced to 50%. METHODS: Breast cancer cells MCF-7 were treated with Triptolide (10-50 nmol/L) for 24 h, and the expression levels of target proteins were detected using Western Blot. RESULTS: Triptolide decreased the messenger RNA and protein levels of ERα in MCF-7 cells in a dose-dependent manner.
• Cell Research:
  Triptolide is dissolved in DMSO (1 mg/mL) and stored, and then diluted with RPMI 1640 medium before use. The viability of differentiated PC12 cells treated with different concentrations of Triptolide. After differentiated PC12 cells are cultured on 96-well plates with RPMI 1640 medium for stabilization, differentiated PC12 cells are incubated with different concentrations of Triptolide (0.01, 0.1, and 1 nM) for 24 hours. The concentrations in this study are chosen. Then cell viability is determined by the MTT assay. Each condition and experiment is repeated three times.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Nuclear factor-κB, Nuclear factor-kappaB, NF-kB, NFκB, NFkB, NF-κB, NSC163062, NSC-163062, NSC 163062, inhibit, Mdm2, Inhibitor, heat shock factor (HSF1)( in ALL cell lines), Apoptosis, Triptolide, PG 490, PG490, PG-490

Compound Identifiers

PubChem CID

107985