TriMethyl-Histone H3-K27 antibody

• Catalog Number: orb137146
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TriMethyl-Histone H3-K27
• Tested applications: ChIP, ICC, IF, IHC, IP, WB
• Reactivity: All, Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic trimethylated peptide around K27 of human histone H3(NP_003520.1).
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200, IP: 1:50-200, ChIP: 1:20-100, ChIP
• Conjugation: Unconjugated
• MW: 17KDa
• UniProt ID: Q16695
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Alternative names: anti HIST1H3J antibody, anti H3/j antibody, anti H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Cheng, Saifeng et al. WOX11 recruits a histone H3K27me3 demethylase to promote gene expression during shoot development in rice Nucleic Acids Res, 46, 2356-2369 (2018)

PubMed: 29361035

Immunohistochemical staining of paraffin embedded rat testis tissue using Histone Histone H3K27me3 antibody (primary antibody dilution at 1:200)

IHC-P image of rat brain tissue using Histone Histone H3K27me3 antibody (primary antibody dilution at 1:200)

IHC-P staining of human thyroid cancer tissue using Histone Histone H3K27me3 antibody (primary antibody dilution at 1:200)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human rectal cancer tissue using Histone Histone H3K27me3 antibody (primary antibody dilution at 1:200)

Immunofluorescense analysis of 293T cell using Histone Histone H3K27me3 antibody

Dot-blot analysis of all sorts of methylation peptides using H3K27me3 antibody

Chromatin Immunoprecipitation analysis of actin gene from 293 cell line TriMethyl-Histone H3-K27 antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli using Histone Histone H3K27me3 antibody

Immunoprecipitation analysis of various genes from 293 cell line using Histone Histone H3K27me3 antibody