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Catalog Number | orb1247238 |
---|---|
Category | Antibodies |
Description | TRIM54 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-GSAGPEEERPDGP |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Experiments gave bands at approx 45kDa and 38kDa in Human Skeletal Muscle lysate after 1ug/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1kDa according to NP_115935, and 38.6kDa according to NP_912730. Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | TRIM54 |
UniProt ID | Q9BYV2 |
NCBI | NP_912730.2, NP_115935.3 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | TRIM54, MURF-3, tripartite motif-containing 54, RN Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Experiments gave bands at approx 45kDa and 38kDa in Human Skeletal Muscle lysate after 1ug/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1kDa according to NP_115935, and 38.6kDa according to NP_912730. Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247238 staining (1 ug/ml) of Human Skeletal Muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
orb1247238 (3.75 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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