TRIM5 antibody

• Catalog Number: orb19133
• Category: Antibodies
• Description: Goat polyclonal antibody to TRIM5
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 0.3 μg/ml, IHC-P: 3-6µg/ml
• Conjugation: Unconjugated
• MW: 56.3
• Target: TRIM5 / RNF88 (alpha isoform)
• Entrez: 85363
• Protein Sequence: CNIEKNENYQPK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TRIM5 antibody, anti tripartite motif-contain
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration, 3-6ug/ml.WB: Preliminary experiments gave an approx 37kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3kDa according to NP_149023.1. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19133 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.