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Catalog Number | orb1247733 |
---|---|
Category | Antibodies |
Description | TRIM3 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Bovine, Canine |
Reactivity | Human, Mouse, Rat |
Immunogen | The immunogen for this antibody is: AKREDSPGPEVQP-C |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 80kDa band observed in Human Brain (Cerebellum) and Rat Brain lysates and approx 85kDa band seen in Mouse Brain (calculated MW of 80.8kDa according to Human NP_006449.2, Mouse NP_001272799.1 and Rat NP_113974.1). Recommended concentration: 0.1-0.3ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | TRIM3 |
UniProt ID | O75382 |
NCBI | NP_150594.2, NP_006449.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | TRIM3, BERP, RNF22, TRIM3, tripartite motif-contai Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 80kDa band observed in Human Brain (Cerebellum) and Rat Brain lysates and approx 85kDa band seen in Mouse Brain (calculated MW of 80.8kDa according to Human NP_006449.2, Mouse NP_001272799.1 and Rat NP_113974.1). Recommended concentration: 0.1-0.3ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247733 staining (0.05 ug/ml) of Mouse Brain lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
orb1247733 (0.3 ug/ml) staining of Human Cerebellum (A) and Rat (B) Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Bovine, Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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