TRIM25 Antibody

• Catalog Number: orb1527669
• Category: Antibodies
• Description: TRIM25 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 325 and 375
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: TRIM25
• UniProt ID: Q14258
• NCBI: NP_005073.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: ubiquitin/ISG15-conjugating enzyme TRIM25; RNF147;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human TRIM25 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, MCF-7, LNCaP, A-549, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIM25 antibody orb1527669 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human TRIM25 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIM25 antibody orb1527669 used for IP at 6 µg per reaction.