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Catalog Number | orb97028 |
---|---|
Category | Antibodies |
Description | TRIF/TICAM1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human TRIF (692-712aa NNHMWNQRGSQAPEDKTQEAE). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 76422 MW |
UniProt ID | Q8IUC6 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | TIR domain-containing adapter molecule 1;TICAM-1;P Read more... |
Note | For research use only |
Application notes | WB: The detection limit for TICAM1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of formalin-fixed paraffin embedded Human Mammary Cancer Tissue using TICAM antibody
Western blot analysis of Lane 1: Recombinant Human TICAM1 Protein 10ng, Lane 2: Recombinant Human TICAM1 Protein 5ng, Lane 3: Recombinant Human TICAM1 Protein 2.5ng, Lane 4: Recombinant Human TICAM1 Protein 1.25ng using TICAM antibody
Western blot analysis of Lane 1: JURKAT Cell Lysate, Lane 2: HL-60 Cell Lysate using TICAM antibody
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