TRIB3 Monoclonal Antibody

• Catalog Number: orb547738
• Category: Antibodies
• Description: TRIB3 Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: AEIE-20
• Tested applications: IP, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human TRIB3 Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity.
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Dilution range: WB 1:1000-1:5000IP 1:20
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 40 kDa
• UniProt ID: Q96RU7
• Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TRIB3 expression in 293T cell lysate using anti-TRIB3 antibody (orb547738). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIB3 antigen affinity purified polyclonal antibody (Catalog # orb547738) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for TRIB3.