TREM2 antibody

• Catalog Number: orb19542
• Category: Antibodies
• Description: Goat polyclonal antibody to TREM2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Human
• Dilution range: ELISA: 1:128000, IHC-P: 2-4 μg/ml
• Conjugation: Unconjugated
• MW: 25.4
• Target: TREM2
• Entrez: 54209
• Protein Sequence: HGQKPGTHPPSELD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TREM2 antibody, anti triggering receptor expr
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Spleen shows staining of macrophages. Recommended concentration, 2-3ug/ml.WB: Preliminary experiments gave bands at approx 80kDa and 30kDa in Human Brain (Cerebellum) lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 25.4kDa according to NP_061838.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Spleen using TREM2 antibody

Western blot of Human Cerebellum using TREM2 antibody